| 質粒名稱: | pXR004: CasRx pre-gRNA cloning backbone |
|---|---|
| 出品公司: | 愛迪基因 |
| 目錄編號: | 109054 |
| 存儲實驗室: | Patrick Hsu |
|
載體骨架基本信息 |
|
| 載體名稱: | pUC19 |
| 載體出品公司: | -- |
| 載體抗性: | 氨芐青霉素 |
| 空載體大小: | 2686 bp |
| 完整質粒大小: | -- |
| 載體修飾: | 插入gRNA表達盒 |
| 載體類型: | 哺乳動物表達,CRISPR |
| 篩選標記: | -- |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | -- |
| 物種來源: | -- |
| 插入大小: | -- |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子 | hU6 |
| 標簽/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位點: | -- |
| 3' 克隆位點: | -- |
| 5' 測序引物 | -- |
| 3' 測序引物 | -- |
|
文獻引用方法 |
|
| 材料和方法部分: | pXR004: CasRx pre-gRNA cloning backbone was a gift from Patrick Hsu (Addgene plasmid # 109054 ; //n2t.net/addgene:109054 ; RRID:Addgene_109054) |
| 參考文獻部分: | Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD. Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033. 10.1016/j.cell.2018.02.033 PubMed 29551272 |
買家導航
pXR004: CasRx pre-gRNA cloning backbone載體質粒基本信息
pXR004: CasRx pre-gRNA cloning backbone質粒圖譜載體圖譜和pXR004: CasRx pre-gRNA cloning backbone載體序列質粒序列多克隆位點信息
pXR004: CasRx pre-gRNA cloning backbone質粒載體簡介
pXR004: CasRx pre-gRNA cloning backbone質粒序列載體序列
LOCUS pXR004: CasRx pre-gRNA cloning backbone 2945 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION hU6-driven expression of guide RNAs compatible with CasRx. Contains
BbsI sites for guide cloning flanked by 5' and 3' full-length DRs.
ACCESSION .
VERSION .
KEYWORDS pXR004: CasRx pre-gRNA cloning backbone
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2945)
AUTHORS Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD
TITLE Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR
Effectors.
JOURNAL Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi:
10.1016/j.cell.2018.02.033.
PUBMED 29551272
REFERENCE 2 (bases 1 to 2945)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..2945
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 7..247
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 7..27
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 178..197
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
rep_origin 428..883
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(515..534)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 725..746
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(900..919)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1019..1041
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1079..1097)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1165..1269
/gene="bla"
/label=AmpR promoter
CDS 1270..2130
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1488..1507)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2301..2889
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2790..2809
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gctagcgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
61 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
121 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
181 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
241 aaaggacgaa acaccgcaag taaaccccta ccaactggtc ggggtttgaa acgggtcttc
301 gagaagacct caagtaaacc cctaccaact ggtcggggtt tgaaactttt tttgaattct
361 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
421 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
481 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
541 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
601 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
661 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
721 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
781 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
841 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
901 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
961 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
1021 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
1081 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
1141 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
1201 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
1261 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
1321 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
1381 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
1441 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
1501 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
1561 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
1621 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
1681 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
1741 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
1801 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
1861 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
1921 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
1981 cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
2041 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
2101 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
2161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
2221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
2281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
2341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
2401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
2461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
2521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
2581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
2641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
2701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
2761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
2821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
2881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
2941 gctca
//